Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Prevalence, Identification and Drug Resistance Pattern of Staphylococcus aureus and Escherichia coli Isolated from Raw Milk Samples of Jaipur City of Rajasthan

Sanjita Sharma, Aarif Khan*, Dinesh Kumar Dahiya, Jyoti Jain and Vishnu Sharma

Advanced Milk Testing Research Laboratory, Post Graduate Institute of Veterinary Education & Research, (Rajasthan University of Veterinary and Animal Sciences, Bikaner), Jaipur - 302020, India.

Received on 06 August 2014 and accepted on 15 October 2014

 

ABSTRACT

The aim of this study was to isolate S. aureus and E. coli from raw milk samples supplied in the Jaipur city of Rajasthan along with their antibiogram patterns. Totally 160 samples were collected from different regions of Jaipur city. After aseptic collection and processing of samples, they were screened by direct plating on selective media viz. Baird-Parker Agar, EMB agar. The presumptive S. aureus and E. coli colonies were confirmed by appropriate biochemical tests. Result revealed that from screened 160 milk samples, 65 isolates (40.63 %) were of S. aureus and 57 (35.63 %) were confirmed as E. coli. Variable antibiotic susceptibility pattern (high to low) were obtained for S. aureus and E. coli isolates against the used drugs. E. coli isolates showed highest resistant towards drug Ampicillin and Penicillin G of 80.76% and 77.19%, respectively. On other side, they were moderately resistance to Nirtofurantoin (42.11%) and Oxacillin (38.60%). In case of obtained S. aureus isolates, highest resistant was noticed to Penicillin-G (86.15%), Cefotaxime (84.62%), Nirtofurantoin (81.54), Ampicillin (73.85%) followed by Chloramphenicol (69.23%) and Tetracycline (64.62%). Present findings clearly point out that the individuals consuming such contaminated raw milk and its products from this region are at high risk of getting ill and might to develop antibiotic resistance.

Keywords : S. aureus, E. coli, Milk, Antibiotic Resistance.