Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Antifungal Activity of Camptothecin Extracted from Mappia foetida against Disease causing Pathogens in Pomegranate (Punica granatum L.)

K.D. Kulkarni1, K.S. Raghuwanshi1*, R.M. Naik2, S.G. Borkar1 and V. P. Chimote3,

1Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth (M.P.K.V.), Rahuri, Ahmednagar - 413722, India. 2Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth (M.P.K.V.), Rahuri, Ahmednagar - 413722, India. 3State Level Biotechnology Centre, Mahatma Phule Krishi Vidyapeeth (M.P.K.V.), Rahuri , Ahmednagar - 413722, India.

Received on 06 September 2014 and accepted on 30 October 2014

 

ABSTRACT

Camptothecin (CPT) is alkaloid known to have medicinal as well as pesticidal properties. Mappia foetida is one of the important source of camptothecin. Therefore an investigation was undertaken with an objective to extract CPT from M. foetida and to evaluate CPT against plant pathogenic fungi and bacteria in pomegranate. High-performance liquid chromatography (HPLC) based analysis revealed the CPT content of roots of M. foetida was 0.18%. Extracted CPT was tested against the leaf spot, wilt and bacterial blight diseases causing pathogens in pomegranate. In vitro bioassays of CPT isolated from M. foetida showed effective control of fungal pathogens like Alternaria alternata, Colletotrichum gloeosporioides and Fusarium oxysporum. Half maximal effective concentration (EC50) of CPT against mycelial growth of A. alternata was 250 ?g/mL, while against C. gloeosporioides it was 500?g/mL CPT. Mycelial growth of F. oxysporum was effectively controlled upto 50 % with 250 ?g/mL CPT. Bacterial blight caused by Xanthomonas axonopodis pv punicae could not be inhibited by CPT at the concentrations tested during in vitro assay.

Keywords : Camptothecin, Bioassay. Alternaria, Colletotrichum, Fusarium, Xanthomonas.