Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Optimization of Poly - Hydroxyl Butyrate (PHB) Production by Bacillus megaterium PTCC 1656

Khosro Issazadeh*, Nasrin Porghanbar Moghadam, Fatemeh Darabipor, Mojgan Hosseini and Narjes Mohammady Bandary

Department of Microbiology, Faculty of Basic Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran.

Received on 25 May 2014 and accepted on 14 July 2014

 

ABSTRACT

Poly ?hydroxyl butyrate( PHB) is a natural,biodegradable polymer accumulated in the form of intracellular granules by a large variety of bacteria. It is among the most investigated biodegradable polymer in recent years. The PHBs are non-toxic, biocompatible and biodegradable thermoplastics that can be produced from renewable resources. The study aimed at screening and identifying a potential PHB accumulating B.megaterium PTCC 1656 and optimization of media parameters for increased PHB production by this strain bioplastics. For this study B.megaterium PTCC 1656 was purchased from the fungi and bacteria collection center of the Iranian science and technology research organization. An their PHB production was determined under different conditions such as incubation time, Carbon and Nitrogen source. The result showed that , glucose was the best carbon source and in this conditions the maximum production of PHB was 0.42 g/l at 45h. PHB production starts in response to stress imposed on cell usually by nitrogen limitation,although in the presence of abundant carbon source. Actually PHB production was dependent on nutrient limitation.

Keywords : Bacillus megaterium, poly-hydroxyl butyrate, optimization.