Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Genetic Diversity and Biocontrol Potential of Rhizospheric Microbes Isolated from Tomato and Maize

Mohammed Aiyaz1, Shetty Thimmappa Divakara1, Santosh Parthasarathy2, Puttaswamy Hariprasad3, Siddaiah Chandra Nayaka1 and Siddapura Ramachandrappa Niranjana1*

1Department of studies in Biotechnology, University of Mysore, Karnataka, India. 2Plant Biotechnology Division, Central Coffee Research Institute, Manasagangotri, Mysore, India. 3Centre for Rural Development Technology, Indian Institute of Technology, Delhi, India.

Received on 06 November 2014 and accepted on 02 January 2015

 

ABSTRACT

Sixteen potential microbial agents consisting of seven bacterial isolates and nine Trichoderma isolates were used in study. Among studied 16 microbial culture isolates Bsp 3/aM, 2apa, Mys 21/a, Has1/c, T2, M5, M29 and M21 showed antagonism against Aspergillus flavus and Fusarium verticillioides and these isolates were positive for root colonization in maize seedlings. Rhizobacterial and Trichoderma sp. diversity was analyzed using sequence and molecular marker based analysis, which failed to group isolates based on antagonism, region of origin and root colonization. Interestingly, in ITS and ISSR diversity analysis two Trichoderma isolates T8 and M5 showed low diversity indicating them to be closely related isolates. The study highlighted use of diverse microbial agents showing antagonism and root colonizing potential which can be used in future for field applications and Screening microbial agents with molecular markers will prevent use of closely related isolates and enhance use of diverse and distinct isolates in biocontrol research.

Keywords : Trichoderma, rhizobacteria, maize, genetic diversity, antagonism and root colonization.