Journal of Pure and Applied MicrobiologyVol. 9 No. 1

The Phytochemicals with Antagonistic Activities Toward Pathogens of a Disease Complex Caused by Meloidogyne incognita and Ralstonia solanacearum

Huang Sheng Zhuo1#, Huang Hui Ni2#, Ma Qing Yun1, Mo Ming He2*, Zhu Ming Liang3, Dai Hao Fu1, Ji Yan Ping4, Wang Qing Hai4 and Zhao You Xing1*

1Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, P. R. China. 2Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, 650091, P. R. China. 3Yunnan of CNTC, Kunming, 650011, P. R. China. 4Shandong Provincial Academy of Forestry, Jinan, 250014, P. R. China.

Received on 09 December 2013 and accepted on 29 January 2014

 

ABSTRACT

Application of phytochemical compounds with dual functional activities against pathogens is a novel strategy to control the disease complexes. Targeting a complex disease of tobacco caused by Meloidogyne incognita and Ralstonia solanacearum, the present study identified four active compounds from stem of a medical plant Daphne acutiloba which with bioactivities against the both pathogens. At a concentration of 25 ?g ml-1, daphneone 2 (1), daphneolon (2), daphnodorin A (3) and daphnodorin B (4) displayed nematicidal activities of 40.23-70.6%, and showed antibacterial circle diameters of 4.04-8.09 mm.

Keywords : Daphne acutiloba; Disease complexes; Phytochemicals; Soilborne pathogens.