Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Propagation of a Field Isolate of Avian Pox Virus on Chorioallantoic Membrane of Developing Chicken Embryo and in BGM-70 Cell Line

R.K. Verma1, R.K. Joshi2, A.K. Gangwar3, Namita Joshi4 and S. Shukla5

1Department of Veterinary Teaching Veterinary Clinical Complex (T.V.C.C), College of Veterinary Science and Animal Husbandry N.D. University of Agriculture and Technology Kumarganj- 224229, Faizabad, India 2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad, India 3Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad, India 4 Department of Veterinary Public Health , College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad - 224229, India. 5Department of Biosciences, Integral University, Lucknow (U.P.) 226026, India

Received on 06 April 2014 and accepted on 19 April 2014

 

ABSTRACT

A field isolate of Fowl pox virus (FPV) was grown successfully on chorioallantoic membrane (CAM) of developing chicken embryo and Baby Gravid Monkey Kidney cell line (BGM-70 cells), as evident by different degree of Cytopathic effects (CPE). Studies were conducted on the pathogenicity of these viruses in these different host systems which produced pock lesions in CAM infected with FPV with extensive congestion and foci of hemorrhages covering entire inoculated site. The cytopathic effects in infected BGM-70 cell monolayers consisted of rounding and clumping of cells, vacuolation, macro and micro plaques and intracytoplasmic inclusion bodies. The cytopathic effects were seen in both unstained as well as MGG stained monolayers of BGM-70 cells

Keywords : Fowlpox virus (FPV), Chorioallantoic membrane (CAM) Pock lesions, Cytopathic effects (CPE), BGM-70