Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Chemical Composition and Biological Activities Guided Assessment of Essential Oils of Two Rutacea Species

Moin Ud Din1, Raja Adil Sarfraz1,2*, Abdullah Ijaz Hussain3, Ijaz Ahmad Bhatti1, Muhammad Shahid4

1Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan. 2Central Hi-Tech Laboratory, University of Agriculture, Faisalabad 38040, Pakistan. 3Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad 38000, Pakistan. 4Department of Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan.

Received on 20 December 2014 and accepted on 07 January 2015



The volatile oil obtained by hydro-distillation of matured peels of Citrus reticulata and leaves of Murraya koenigii, belonging to the family Rutacea were subjected to GC-MS analysis for their chemical characterization. Major components were found to be Limonene (92.83%) in C. reticulata and Eugenol (81.61%) in M. koenigii. Both oils showed good antioxidant activity by scavenging DPPH radical (IC50 = 24.77 and 26.68 ?g/ml) and inhibiting linoleic acid (70.33 and 82.78 %) for both C. reticulata and M. koenigii, respectively. Antimicrobial activity of oils was accomplished using disc diffusion method followed by minimum inhibitory concentration (MIC) against various pathogenic bacteria and fungi. Fungal strains were found to be more sensitive against both of the oils. Minimum hemolysis (0.08 & 0.34%) was observed at 0.5 mg/mlin case of M. koenigii against both human and bovine erythrocytes, respectively.

Keywords : Citrus reticulata, Murraya koenigii,GC-MS, Antioxidant activity, antimicrobial activity, Hemolytic Assay