Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Mollicutes Isolates and their Relationship to Infertility in Women

A. Rivera1*, L. Cedillo1, O. Romero1, C. Gil1, N. Rodriguez2 and S. Giono3

1Mycoplasma Laboratory Research Center in Microbiological Sciences, Institute of Sciences of the Autonomous University of Puebla. Mexico. 2Mycoplasmas National Laboratory, Pedro Kouri Institute, Havana, Cuba. 3Laboratory of Medical Microbiology, National School of Biological Sciences, National Polytechnic Institute. Mexico, D.F.

Received on 10 December 2014 and accepted on 26 January 2015

 

ABSTRACT

`Genital mollicutes are considered a human pathogen of great importance as sexually transmitted agents and are involved in a variety of infectious such as urethritis, prostatitis, bacterial vaginitis and other inflammatory processes which leads to infertility. The objective was the isolation of mollicutes from vaginal swabs and establish its relationship to infertility in women. Two hundred fifty vaginal swabs were included, processing by microbiological culture and PCR, and data from the clinical history of the patients were analyzed in order to confirm their relationship of isolates with infertility. Of the 250 women examined, the total positive rates of ureaplasma and mycoplasma species for the 28-32 years old were the 20 %, while for the elder female being 48-52 year sold. The assay PCR-amplified products from urea plasma and mycoplasma of 429 bp and 301 bp, respectively. Some damage mechanisms involved in the infections by pathogenic organism such as genital mollicutes that affect or interrupt the fertilization process are oxidative stress, damage mechanism via receptor, membrane enzymes and DNA fragmentation. Ureaplasma spp., and Mycoplasma spp., is associated with several diseases of women of reproductive age, as urethritis, urinary tract, infections, chorioamnionitis, spontaneous abortions, pelvic inflammatory disease and infertility. In Mexico, are recognized as two of the major microbial genera isolated from patients diagnosed with in fertility.

Keywords : Diagnostics, mycoplasma, ureaplasma, infertility, women.