Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Biological Control of Postharvest Diseases of Chestnut Fruit by Microbial Antagonists-Endophytic Bacteria CE3

Li Linling1,3, Cheng Hua1,3, Hua Juan1,3, Yuan Honghui1,3, Chen Xiaoling1 and Cheng Shuiyuan1,2*

1Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang, Hubei, China. 2School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China. 3College of life science, Huanggang Normal University, Huanggang, Hubei, China.

Received on 10 December 2014 and accepted on 15 January 2015

 

ABSTRACT

24 endophytic strains were isolated from healthy Luotian chestnut tissue in China. 22 strains among the above 24 ones were bacterium, other strains were fungi. CE2, CE3 and CE8 showed effective biocontrol activities through flat confrontation method. Further, among the 3 strains CE3 had the best antagonistic effect. The inhibition zone width was 10.74 mm, 10.14 mm, 11.28 mm to Cryphonectria parasitica, Penicillium expansum and Fusarium solani. Based on the morphological, physiological characteristics and 16S rDNA sequence analysis, the strain CE3 was identified as Bacillus cereus. It?s obvious that CE3 has the best inhibitory effect before infection of pathogenic bacteria, the best concentration is 109 CFU ml-1. In order to enhance the bacteriostatic effect of B. cereus, we made a test, which was the B. cereus combine with auxiliary factor. The results showed that, When CaC12 and MgC12 were used in conjunction with CE3, there was significantly better control effect on P. expansum than individual uses of two adjuvants. The effect of treatment with CE3 alone had no significant difference. B. cereus CE3 controlled the growth of pathogenic fungi was enhanced by the addition of CaC12, MgC12. In addition, a combined use of carbendazim that had been diluted for 200,000 times and CE3 enabled the incidence rate of A. niger to drop significantly, with better inhibitory effect than exclusive use of CE3 or low doses of carbendazim.

Keywords : Castanea mollissima Blume; Endophyte bacteria; Biological control; Isolation and identification.