Journal of Pure and Applied MicrobiologyVol. 9 No. 2

Antimicrobial Activity of Silver Nanoparticles Synthesized by Reduction Technique

Hamid Reza Ghorbani*?, Roya Rashidi?

?Department of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran. ?Department of Chemical Engineering, Tehran North Branch, Islamic Azad University, Tehran, Iran.

Received on 12 April 2015 and accepted on 28 May 2015

 

ABSTRACT

Silver metals have long been known to possess antimicrobial properties. Recently, Ag nanoparticles have also been proven as antimicrobials. In this work, Ag nanopartilces were synthesized by hydrazine hydrate. UV?vis absorption spectra, dynamic light scattering (DLS) and transmission electron microscopy (TEM) have been used to analysis the synthesis of silver nanoparticles. The antimicrobial activity of the Ag nanopartilces was tested against pathogenic Escherichia coli. The mechanism of Ag nanopartilces against E. coli may attribute to Ag nanopartilces leads to cell wall lysis and damages cell membrane integrity.

Keywords : Antimicrobial, Silver nanoparticles, Escherichia coli, Chemical synthesis.