Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Uropathogenic Escherichia coli in Diabetic Patients of Iran; Virulence Factors and Antibiotic Resistance Properties

Maryam Dadmanesh1, Adel Hamidi2, Maryam Eramabadi3 and Mahsa Faghihi4*

1Department of Infectious Diseases, School of Medicine, AJA University of Medical Sciences,Tehran , Iran 2Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, Karaj, Iran. 3Young Researchers and Elite Club, Chalous Branch, Islamic Azad University, Chalous, Iran. 4Department of Medical Biotechnology , Qazvin University of Medical Sciences , Qazvin , Iran

Received on 18 October 2014 and accepted on 06 December 2014

 

ABSTRACT

From a clinical prospective, it is important to know the distribution of virulence factors and antimicrobial resistance properties of Uropathogenic Escherichia coli isolated from diabetic patients with severe urinary tract infections. Therefore, the present study was carried out in order to reach above goals. Totally, 110 urine samples were collected from diabetic patients hospitalized due to UTIs. Samples were cultured and those that were E. coli positive were subjected to PCR assays to study the distribution of virulence factors. Simple disk diffusion was carried out to study the antibiotic resistance pattern of E. coli isolates. We found that 33 out of 110 diabetic urine samples (30%) were positive for E. coli. E. coli strains of diabetic patients had the highest levels of resistance to tetracycline (87.8%), ampicillin (84.8%), gentamycin (84.8%), ciprofloxacin (75.7%) and cephalothin (54.5%). In the other hand, imipenem and mezlocillin were the best choices for treatment of the cases of UTIs in diabetic patients. Sfa (45.4%), fim (45.4%) and afa (36.3%) had the highest incidence among virulence factors while, usp (3.0%), sigA (6.0%) and iha (6.0%) had the lowest incidence. Conclusions: Virulent strains of uropathogenic E. coli were predominant cause of UTIs in diabetic patients of Iran. High hygienic care, treatment of diabetes and using from disk diffusion method can reduce the dangerous risk of uropathogenic E. coli in diabetic patients.

Keywords : Diabetic patients, Urinary tract infections, Uropathogenic Escherichia coli, Virulence factors, Antimicrobial susceptibility.