Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Optimization of Process Parameters for Erythromycin Production Under Solid State Fermentation by Saccharopolyspora erythraea NCIMB 12462

Azza M. Noor El Deen1, Nayera A.M. Abdelwahed1*, Hoda M.A. Shata2 and Mohamed A.M. Farid1

1Chemistry of Natural and Microbial Products Department, National Research Centre, 12622, Dokki, Giza, Egypt. 2Department of Microbial Chemistry, National Research Centre, 12622, Dokki, Giza, Egypt.

Received on 06 December 2014 and accepted on 17 January 2015



Solid-state fermentation (SSF) was conducted on laboratory scale in 250 ml Erlenmeyer flask at 30C for erythromycin production by Saccharopolyspora erythraea NCIMB 12462. Several agro-industrial wastes were selected and screened in order to determine the most suitable one for the production of erythromycin by the above mentioned microorganism. Of all the substrates evaluated, sugarcane bagasse was found above mentioned to be the best. Water was the best eluent for the extraction of the antibiotic from the solid-state culture compared with other organic solvents yielded about 197.913.11 g/g solid .The cultural conditions were optimized such as inoculum size of 4 ml vegetative culture supported a maximum erythromycin production of 197.9715.52 g erythromycin /g solid, moisture content of 85% culture resulted in a maximum production of 308.48.2 g/g . Furthermore , the weight of sugarcane bagasse of 2.5 g gave 41432.5 g/g solid. After medium optimization the production was enhanced to 416.416.55 g /g solid. Through the incubation time, erythromycin detection started on the 3rd day and attained its maximum level of 600.6547.1 g/g solid on the 10th day.

Keywords : Solid state fermentation, Erythromycin, Sugarcane bagasse, Saccharopolyspora erythraea NCIMB 12462