Suman Abhishek1, Anil Kumar Gupta2, Anjuvan Singh3

1Department of Biotechnology, Indian Institute of Technology Hyderabad, Hyderabad, Telangana, India
2Microbiology Division, Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research), Patna, Bihar, India
3Department of Biotechnology, School of Biotechnology and Biosciences, Lovely Professional University, Phagwara, Punjab, India

DOI: http://dx.doi.org/10.22207/JPAM.11.4.09

(Received: 01 October 2017; accepted: 01 November 2017)

Abstract:

Heme, a Fe-centred hexacoordinatedorganometallic compound mainly present in blood proteins, acts as a major source of iron (Fe2+) for various hemolytic microorganisms.This makesblood an essential mediumsupplement for characterizing such organisms. Considering theincreasing blood demand for this purpose, we are proposing an alternative approach for hemolytic characterization of bacteria using plant derivative. For the first time, we present the kinetic model of growth of Staphylococcus aureus, a beta-hemolytic, gram-positive rod-shaped bacterium, and subsequent hemeuptake at anoptimized concentrationof crude proteins of Beta vulgaris(red beetroot). In this paper, we have determined the heme content of the beetroot juice and demonstrated the ability of S. aureus to uptake heme from beetroot protein solution.According to the determined growth kinetics, the doubling time of the bacterium in the beetroot protein supplemented medium was 25 minutes. The heme uptake analysis showed a zeroth order kinetics with a constant decline rate of 0.19μM h-1.

Keywords:

Plant heme, 3R’s, Beetroot, Hemolytic characterization, Heme uptake kinetics, Growth kinetics