K.B. Rudrappa1, A.P. Suryawanshi, N.D. Punitkumar., J.K. Ganesh3, Kumar Lambani3 and Roop Singh1

1Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani- 431402, India. 2Technical Assistant, Dean PGS, University of Agriculture Horticultural Sciences, Shivamoga. 3Department Plant Pathology University of Agricultural Science, Dharwad

Received on 29 March 2016 and accepted on 21 May 2016

 

ABSTRACT

Cultural characteristics viz., colony count, colony colour and colony shape of Ralstonia solanacearum on different period of time were studied in vitro using eight culture media. Highest average colony count was recorded on triphenyl tetrazolium chloride agar (65.33), white fluidal colonies with spiral pink centre and the minimum average colony count was recorded on yeast extract chalk agar (41.50) with irregular yellow color colonies. The bacterium showed positive reaction for solubility in KOH, production of catalase, motility test, hydrolysis of starch & casein and negative reaction for gram staining.

Keywords :

 Ralstonia solanacearum, culture media, biochemical characteristics and Solanum lycopersicum.