H.M. Renuka, H.K. Ramappa and B.S. Pavithra

Department of Plant Pathology, College of Agriculture UAS, GKVK, Bengaluru – 560 065, India.

Received on 14 May 2016 and accepted on 27 June 2016

 

ABSTRACT

Field bean (Lablab purpureus (L.) Sweet) is one of the important pulse crops in tropics. Dolichos mosaic virus (DMV) infected field bean plants produced symptoms like vein clearing, uneven leaf lamina, twisting of leaves, mosaic mottling, puckering and blistering on newly formed trifoliate leaves. The purified preparation of the virus under electron microscopy revealed the presence of long flexuous rod shaped particles approximately measuring ~750 nm in size. RT-PCR product ~ 340 bp amplified by using BCMV specific partial CP gene primers indicated the infection of BCMV in dolichos.

Keywords

 Lablab purpureus (L.), Puckering, DMV, BCMV and Electron microscopy.